Folding and stretching in a Go-like model of titin

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Folding and stretching in a Go-like model of titin.

Mechanical stretching of the I27 domain of titin and of its double and triple repeats are studied through molecular dynamics simulations of a Go-like model with Lennard-Jones contact interactions. We provide a thorough characterization of the system and correlate the sequencing of the folding and unraveling events with each other and with the contact order. The roles of cantilever stiffness and...

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Thermal effects in stretching of Go-like models of titin and secondary structures.

The effect of temperature on mechanical unfolding of proteins is studied using a Go-like model with a realistic contact map and Lennard-Jones contact interactions. The behavior of the I27 domain of titin and its serial repeats is contrasted to that of simple secondary structures. In all cases, thermal fluctuations accelerate the unraveling process, decreasing the unfolding force nearly linearly...

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Mechanical properties of the domains of titin in a Go-like model.

Comparison of properties of three domains of titin, I1, I27, and I28, in a simple geometry-based model shows that despite a high structural homology between their native states different domains show similar but distinguishable mechanical properties. Folding properties of the separate domains are predicted to be diversified which reflects sensitivity of the kinetics to the details of native str...

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Mechanical Stretching of Proteins: Calmodulin and Titin

Mechanical unfolding of several domains of calmodulin and titin is studied using a Go-like model with a realistic contact map and Lennard-Jones contact interactions. It is shown that this simple model captures the experimentally observed difference between the two proteins: titin is a spring that is tough and strong whereas calmodulin acts like a weak spring with featureless force-displacement ...

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Sequencing of folding events in Go-like proteins

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ژورنال

عنوان ژورنال: Proteins: Structure, Function, and Genetics

سال: 2002

ISSN: 0887-3585,1097-0134

DOI: 10.1002/prot.10087